For further information, please contact: Sally Wang Executive Vice President GenScript 1-(732) 885-9188 Fast Gene Synthesis. DNA synthesis techniques and technologies are quickly becoming a cornerstone of modern molecular biology and play a pivotal role in the field of synthetic. 🎎 We will exhibit at BioJapan 2023! At this exhibition, we will showcase our strength in artificial gene synthesis to mRNA production, protein. Complex. 金斯瑞GenBrick™长片段基因合成服务可为您合成长达8-15 kb的长片段基因, 合成序列无任何突变或错误,保证序列100%准确。该服务将以更为实惠的价格,23个工作日即可交付您所需的基因。DNA primase forms an RNA primer, and DNA polymerase extends the DNA strand from the RNA primer. B. We spoke with Cedric Wu, Ph. D. Gene synthesis is a timely and cost-effective solution to traditional molecular cloning. GenScript has launched an upgrade to its GenBrick Gene Synthesis platform, which gives researchers access to DNA sequences up to 200 kilobases long with 100 percent accuracy. Inquire today about our newest service! Gene Services. right from the start. 5 Mb sections of the synthetic genome, starting from episomes bearing 100 kb fragments of synthetic. 23/bp $0. In biology, the word gene (from Greek: γένος, génos; [1] meaning generation [2] or birth [1] or gender) can have several different meanings. Role of the hemA gene product and δ-aminolevulinic acid in regulation of Escherichia coli heme synthesis. SC1619 Cat No. D. Click on the vector name to view the full vector map. prevents the initiation of transcription of the DNA sequence that codes for ATP synthase. 1950 DNA polymerase isolated from E. Service enables synthesis of DNA sequences up to 200kb with 100 percent sequence accuracy . Figure 1. GeneStrands; Express GeneStrands; Molecular Biology Services. Gene synthesis is an international business, with companies based all over the world, so oversight and monitoring is complicated. 0 with ease . 23/bp $0. First strand synthesis: First, one anneals an oligo dT primer onto the 3' polyA tail of a. Stage 1: Initiation. Some 50 different protein transcription factors bind to promoter sites, usually on the 5′ side of the gene to be transcribed. Containing two chains (A and B) connected by disulfide bonds, the mature hormone is the post-translational product of a single-chain precursor, designated. A first combinatorial reaction cocktail is used for assembly of gene module, while a second reaction is used for generation of two-gene module from individual gene module in plasmid 2. SC1010 Rush Gene Synthesis Cat No. SC1681 GenBrickTM Synthesis Cat No. Applied Research Laboratories, The University of Texas at Austin, Austin, Texas, USA. The solid phase synthesis segment accounted for a dominant market share in 2021. Express Cloning. SC1584 $0. In this chapter, we address genome replication strategies including the diverse strategies that exploit the biology of the hosts, control gene expression, and ensure preferential. From applications like gene synthesis, genetic diagnostic test kits, PCR/RT-PCR, siRNA/RNAi, miRNA, CRISPR Cas9, and DNA/RNA. Custom Molecular Clones. Sequences greater than 5 kb can be reviewed for acceptance as a custom project. D. SC1681 GenBrickTM Synthesis Cat No. deletion. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. They're all designed to help ensure that the time you invest in your research is efficient and is rewarded with. Here we introduce a software tool that drastically facilitates the design of synthetic genes. SC1645 GenPlusTM Economy Gene Synthesis Cat No. It is frequently far faster, straightforward, and less costly than using alternative methods of molecular cloning and. Protein synthesis involves a complex interplay of many macromolecules. 35/bp Request a quote Request a quote $0. Gene Synthesis; High-Throughput DNA Library Assembly; Catalog IVT mRNA and circRNA Products; Custom RNA Oligo; Peptide Synthesis Services; CRISPR Plasmids;. (2021). No. The amino terminus of a polypeptide will contain amino acides whose codons are closer to the ___ end of the mRNA. synthesis Day 2 Assembly Day 3 Day 4 Day 5 Cloning into vector QC by multiple methods Delivery of construct with gene of interest . USA 100, 12390–12395. You may contact us for quotation requests by email , phone, fax, or via our secured online quotation system or by email at gene@genscript. Key advances in DNA sequencing and gene synthesis are at the base of tremendous progress in tailoring biocatalysts by protein engineering and design, and the ability to reorganize enzymes into new. GenScript Launches Enhanced GenBrick Gene Synthesis Service for Building Synthetic Genomes. Two ferrochelatase mRNA species, approximately 2. 23/bp $0. a. Custom Gene Synthesis Fast Gene Synthesis Cat. 7. SC1681 GenBrickTM Synthesis Cat No. , GenScript Sr. GenScript Asia Pacific. and even genes longer than 8 kb through GenBrick™ Gene. Pairs with GeneArt seamless cloning kits to assemble your complete gene. 45/bp. 6. Number of genes. Service enables synthesis of DNA sequences up to 200kb. During the initial step of λ DNA replication, the phage replicates using the θ mode to produce multiple copies of circular DNA. Gene Synthesis is a reliable and cost-effective method for obtaining customized DNA constructs with 100% sequence accuracy. Select the type of oligo—DNA or RNA—to be analyzed. No. No. We have previously constructed this opron using gnomic DNA and subcloning but. Proc. com. Biomatik has been proudly serving the life sciences and drug discovery community since 2002. GO enrichment analysis revealed that protein synthesis, organonitrogen compound biosynthetic and metabolic process, and endoplasmic reticulum-related genes. 3d. Our Poly A Guarantee service combines GenScript’s gene synthesis expertise with industry know-how to provide high. C) corepressor binding to the operator. Invitrogen cell, protein, and molecular biology technologies range from Lipofectamine reagents, TOPO cloning kits, SuperScript and Platinum enzymes, to western blotting technologies, antibodies, and the GeneArt Gene Synthesis service. siamense infection, which were conducted for. The whole-genome duplication 80 million years ago of the common ancestor of salmonids (salmonid-specific fourth vertebrate whole-genome duplication, Ss4R). Optimal for mid-scale pathway screening, mini. SC1681 GenBrickTM Synthesis Cat No. No. 2). Thermo Fisher Scientific: GenBrick: GenBrick is synthesized genetic fragments ranging between 8 and 15 kb; can be useful for synthetic genomes, metabolic engineering, industrial microbiology, etc. 2011;498:277-309. As part of its structure, a known gene contains the base sequence AATCGA. In this work, the xylem microstructure of. The first stage of small molecule inhibitor identification requires large-scale screening against proteins involved in these pathways. 23/bp $0. No. Visit our booth at C-16 to learn more about our capabilities in gene synthesis, antibody production, mRNA and cGMP related services!. SC1681 GenBrickTM Synthesis Cat No. As a powerful molecular biology tool, DNA synthesis is rapidly becoming a crucial part of research workflow and has transformed modern biology. 45/bp. Economy gene synthesis offers low-cost gene synthesis starting at $0. 23/bp $0. 23/bp $0. 4% = 0. Predesigned and custom assays can be provided in tubes or. 26 Transcription: from DNA to mRNA In the first of the two stages of making protein from DNA, a gene on the DNA molecule is transcribed into a complementary mRNA molecule. Cloning, Mutagenesis and Plasmid Prep. Custom Gene Synthesis Fast Gene Synthesis Cat. This multi-functional tool facilitates the theoretical calculation of several oligo sequence dependent properties. Custom Gene Synthesis Fast Gene Synthesis Cat. Unlike DNA synthesis in living cells, artificial gene synthesis does not require template DNA, allowing virtually any DNA sequence to be synthesized in the laboratory. With our proprietary algorithm, GenSmart is a free online interface that makes it possible to design and price your optimal sequence in a single place. Sci. Manufacturing one gene using the Twist method is equivalent to the emissions from driving a gas car 0. This solution will become increasingly attractive as the cost of gene synthesis continues to decrease. RNA contains uracil in place of thymine. No. 23/bp $0. Restriction synthesis is a novel synthetic deoxyribonucleic acid (DNA) synthesis method. 35/bp Request a quote Request a quote $0. Gblocks are a double stranded sequence of DNA, constructed by a synthesis company. 6. Gene Symbol Full Name Gene Type + TFPI tissue factor pathway inhibitor: protein-coding + WFDC12 WAP four-disulfide core domain 12: protein-coding + SERPINB12 serpin family B member 12If gene synthesis transitioned to array-based oligos, there are no prima facie reasons why costs could not fall 3–5 orders of magnitude to be on par with the cost of oligo pools ($1 per 10 3. Ribosomes: The eukaryotic ribosome has two subunits: a 40S small subunit and a 60S large subunit. Custom any ORF cDNA clone into your choice vector. We spoke with Cedric Wu, Ph. 23/bp $0. SC1681 GenBrickTM Synthesis Cat No. GenScript has launched an upgrade to its GenBrick Gene Synthesis platform, which gives researchers access to DNA sequences up to 200 kilobases long. 3'. The nucleotide sequence of a gene is ultimately translated into an amino acid sequence of the gene’s corresponding protein. The complete set of correspondences between codons and amino acids (or stop signals) is known as the genetic code. The translation is illustrated in Figure 6. It has raised $30. This kinase integrates multiple stimuli coming not only from hormones but also from. It requires breaking the DNA of the. combine with gene synthesis, custom cloning, or mutagenesis to simplify your research workflow. For over 60 years, A. 45/bp. Custom Gene Synthesis Fast Gene Synthesis Cat. Synthesis. 23/bp $0. ASM Objective: 04. Gene modules of Turquoise and Salmon, containing 1039 and 139 respectively, were associated with C. Each ORF cDNA clone in our clonesets are fully sequence verified and. Home » Gene Synthesis & Molecular Biology Services » Plasmid DNA Preparation » Research Grade Plasmid DNA Preparation Overview GenScript's research grade plasmid DNA can help your on standard preps for cloning, mutagenesis and transformation , from microgram- to gram-scale with reliable quality and fast turnaround time to accommodate tight. Metabolism. 23/bp $0. changes a stop codon to a codon specifying an amino acid. Like DNA, RNA is made up of nucleotide consisting of a 5-carbon sugar ribose, a phosphate group, and a nitrogenous base. No. 23/bp $0. Hablamos con Cedric Wu, Ph. Many gene synthesis companies now perform self-governance, screening DNA orders for known dangerous sequences. 45/bp. 23/bp $0. More importantly, we've engineered the labor out of the install, so GenStone is DIY friendly and costs about 50% less than masonry work. SC1645 GenPlusTM Economy Gene Synthesis Cat No. 20,577 followers. synthesis Day 2 Assembly Day 3 Day 4 Day 5 Cloning into vector QC by multiple methods Delivery of construct with gene of interest . Figure 3. Gene Synthesis Handbook. However, assembling long sequences can prove problematic and slow down the process of gene assembly. We spoke with Cedric Wu, Ph. GenPlus Economy Service. J. SC1010 Rush Gene Synthesis Cat No. No. Find the right expression vector for your ORF clone from our comprehensive list of vectors organized by expression host, promoter, bacterial selection, copy number, and epitope tag. "As a pioneer in gene synthesis, we are proud to add very long DNA sequences to our. When genes are expressed, they produce _____. 12 per bp) and can take up to 3 weeks to obtain. Genssript; Evaluating online and offline accuracy traversal algorithms for k-complete neural network [email protected] Information. Tufts University & Harvard. J. During this process, the DNA sequence of a gene is copied into RNA. Up to 80% off! Identification of small molecule inhibitors of disease-related pathways is one of the most important elements of the drug development process. 4 using TOP10 competent cells. SC1645 GenPlusTM Economy Gene Synthesis Cat No. 2 3. Bioscience is a leading and rapidly growing synthetic biology and genomics company that has developed a disruptive DNA synthesis platform to industrialize the engineering of biology. SC1645 GenPlusTM Economy Gene Synthesis Cat No. SC1619 Cat No. The translation is the second part of the central dogma of molecular biology: RNA --> Protein. A gene synthesis project has both wet and dry lab components. Arthur Kornberg wins 1957 Nobel. Gene Synthesis Handbook - GenScript - Make Research EasyGenScript Asia Pacific. b. Today, with the right equipment and materials, sequencing a short piece of DNA is relatively straightforward. SC1645 GenPlusTM Economy Gene Synthesis Cat No. 23/bp $0. Plasmid Preparation; CRISPR/Cas9 >> Show more. The inhibition of cholesterol synthesis and esterification is dependent, in part, on the upregulation of the interferon-stimulated gene, Ch25h, and the production of oxysterol 25HC. Like DNA replication, there are three stages to transcription: initiation, elongation, and termination. Biomatik has been proudly serving 10,000+ scientists since 2002. In November 2021, Codex DNA, Inc. Number of genes: 6098. SC1584 $0. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. 23/bp $0. However, to make these two applications more practical,. Craig Venter. Genes achieve their effects by directing the synthesis of proteins. PISCATAWAY, N. 5 kb and approximately 1. SC1645 GenPlusTM Economy Gene Synthesis Cat No. and even genes longer than 8 kb through GenBrick™ Gene Synthesis. GeneArt gene synthesis provides synthesis and cloning of any desired genetic sequence ready for any downstream application. GenScript’s gene to mRNA end-to-end solution offers high quality optimized IVT templates to deliver high integrity mRNA for exceptional protein expression. d. SC1645 GenPlusTM Economy Gene Synthesis Cat No. Gene Synthesis. Learn more about our GenBrick™ technologies for long gene synthesis. SC1010 Rush Gene Synthesis Cat No. The Genetic Code: A Universal Template for Protein Translation. GenBrick™ long fragment gene. SC1619), and Economy (Cat No. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. SC1584 $0. forms a new stop codon. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. Gene Synthesis Provider: Express Gene Synthesis – Fastest turnaround; GenBrick™ Synthesis – up to 200 kb DNA sequence; GenParts™ DNA Fragments; Precision Mutant Libraries; Combinatorial DNA Library Assembly; KO/KI/RNAi Vector Construction; Oligo Pools and NGS Total Solutions. 🎎 We will exhibit at BioJapan 2023! At this exhibition, we will showcase our strength in artificial gene synthesis to mRNA production, protein. Overview. 45/bp. Nevertheless, unlike ACC1, this ACC2 gene seems to be dispensable in Arabidopsis (Babiychuk et al. Download our Gene Synthesis Handbook and explore our resource links to accelerate your research. As one of the leading gene synthesis companies, GenScript can synthesize more than 30,000 genes per month, with the longest synthetic gene fragment up to 200 kb. mRNA Synthesis New! Gene to Lentivirus One Stop Package New! IVT mRNA Production New! Gene Synthesis Upgraded; Gene Synthesis; Gene Fragments New! Plasmid DNA Preparation Upgraded; Plasmid DNA Preparation; Cloning and Subcloning; ORF cDNA Clones ; Custom Vector Construction; GenCircle™ Double-Stranded DNA New！. Although different types of collagen may undergo different post-translational modifications, the basic outline for collagen synthesis is listed below. D. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. methionine. With our proprietary silicon-arrayed production process, the chip-based platform can synthesize over 7,000 genes in a single run. Section: 09. Our custom gene synthesis services are designed to meet your specific requirements without compromising on quality. SC1010 Rush Gene Synthesis Cat No. The use of 3-letter codons in mRNA means that 64 possible codons encode a pool of 20 amino acids and translation stop signals. Gene Synthesis; mRNA Synthesis New! Gene to Lentivirus One Stop Package New! IVT mRNA Production as fast as 2 weeks; Gene Synthesis; Plasmid Preparation; Cloning and Subcloning; ORF cDNA Clones ; Custom Vector Construction; IVT mRNA Production New！ GenCircle™ Double-Stranded DNA New！ GenSmart™ Online Tools; Gene Variant. 35/bp Request a quote Request a quote $0. coli. Rare Codon Analysis Tool. Page. Gene Synthesis Upgraded; Gene Synthesis. 23/bp $0. Most newly synthesized RNAs must be modified in various ways to be converted to their functional forms. 35/bp Request a quote Request a quote $0. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. 35/bp Request a quote Request a quote $0. Quite often, we just do not know enough. SC1584 $0. Each gene stores the directions for. 23/bp $0. With offerings at 10 μg and 30 μg quantities, you'll now be able to economically order multiple plasmid preps, all with our stringent quality control measures. Life Science products & services and biologics services | GenScript Biotech (Singapore) Pte. PISCATAWAY, New Jersey, Jan 31st, 2013 – GenScript Incorporation has launched the e Blot ® Protein Transfer System, a semi-dry electric blotter for transferring proteins from mini polyacrylamide gels onto membranes, expanding its bench-top tool offerings for protein analysis. Insulin plays a central role in the regulation of human metabolism. 45/bp. 20,577 followers. With our proprietary silicon-arrayed production process, the chip-based platform can synthesize over 7,000 genes in a single run. 35/bp Request a quote Request a quote $0. Ordering genes or gene fragments from a gene synthesis company is an alternative to cloning of level 0 basic parts from PCR products. Building on GenScript's expertise in molecular biology and gene. 15 kilometers 2. VectorArk™ cloning is available with Regular (Cat No. We now know that each type of antibody chain—κ light chains, λ light chains, and heavy chains—has a separate pool of gene segments and exons from which a single polypeptide chain is eventually synthesized. No. attachment of an amino acid to tRNA. RNA serves as the template in cDNA synthesis. The nucleus. Different types of media can also be tested; rich media, such as Terrific Broth, 2×YT or ZYP5052 (auto-induction. These chromosomes are made up of thousands of shorter segments of DNA, called genes. Drug development (target identification, advancing drug leads to candidates for preclinical and clinical studies) can be facilitated by genetic and genomic knowledge. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. FLJ92943) Ensembl ID (e. 1. You will be able to select your vector for custom cloning after selecting your gene. Optimal for pathway screening, GenScript's ORF cDNA clonesets can be purchased as single clone selection or as individual clones that you choose. SC1010), Fast (Cat No. , λ) to replicate double-stranded DNA. 35/bp Request a quote Request a quote $0. SC1619 Cat No. Quantitative genomic DNA measurement by qPCR is available with additional charges. Custom Gene Synthesis Fast Gene Synthesis Cat. DNA synthesis. 2. 2: Transcription: from DNA to mRNA. SC1010 Rush Gene Synthesis Cat No. Synthetically constructed genes come by many names, such as Gblock, Oligonucleotide or Gene Fragment. It stores instructions for making other large molecules, called proteins. formation of ribosomal subunits. Normally, its unpaired nucleotides are "read" by transfer RNA anticodons as the ribosome proceeds to translate the message. Genssript; Evaluating online and offline accuracy traversal algorithms for k-complete neural network architectures. VectorArk™ Cloning. GenBrick™ utilizes a novel proprietary technology to enable accurate one-step DNA assembly. 45/bp. GenScript®, a world leading gene synthesis provider, hopes to combat some of the challenges associated with long DNA sequence assembly through an upgrade to its GenBrick™ Gene Synthesis platform. and even genes longer than 8 kb through GenBrick™ Gene Synthesis. Our cost-effective chip-based platform makes it possible for every research lab to get custom. Bundled with gene synthesis: VectorArk™ Cloning Bundled with gene synthesis Subcloning Bundled with gene synthesis Service Number SC1691 SC1692 SC1017 Advantages: Expression vectors – Choose from over 150 popular vectors listed: Save on cloning into your archived vectors: Get ready to use clones in any vector, commercial or. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99. No. 45/bp. Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. Current challenges. [Codon table] → → → →. SC1645 GenPlusTM Economy Gene Synthesis Cat No. SC1619 Cat No. 45/bp. The carboxyl terminus of a polypeptide will contain amino acides whose codons are closer to the ___ end of the mRNA. com . It has also developed new. Gene Synthesis; mRNA Synthesis New! Gene to Lentivirus One Stop Package New! IVT mRNA Production as fast as 2 weeks; Gene Synthesis; Plasmid Preparation; Cloning and Subcloning; ORF cDNA Clones ; Custom Vector Construction; IVT mRNA Production New！ GenCircle™ Double-Stranded DNA New！ GenSmart™ Online Tools; Gene Variant. SC1645 GenPlusTM Economy Gene Synthesis Cat No. SC1010 Rush Gene Synthesis Cat No. SC1645). It can be broken down into the following steps: Define project goals; Design the sequence; Synthesize oligos; Assemble oligos into linear fragments and larger constructs (if applicable) Verify the sequence of the gene fragment or cloned product 1. structural locus. Custom Gene Synthesis Fast Gene Synthesis Cat. D. Scientists have begun attempts to synthesize living organisms from scratch instead of solely modifying the genome of a living organism. It may lead to new intellectual progress. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. SC1645 GenPlusTM Economy Gene Synthesis Cat No. Science, 230 (4723), 281–285. Complex. Also, in basic research, the fusion gene fragments are used to identify the gene regulatory sites in cells [27]. 45/bp. Offered Products: 31,000+ ELISA Kits, 14,000+ Antibodies, 17,000+ Proteins, 230+ Biochemicals. SC1619 Cat No. SC1645 GenPlusTM Economy Gene Synthesis Cat No. SC1681 GenBrickTM Synthesis Cat No. Gene Synthesis is a reliable and cost-effective method for obtaining customized DNA constructs with 100% sequence accuracy. SC1619), and Economy (Cat No. Economy gene synthesis offers low-cost gene synthesis starting at $0. :Catalpa bungei is an economically important tree with high-quality wood and highly valuable to the study of wood formation. Custom Gene Synthesis Fast Gene Synthesis Cat. proline. g. Plasmid. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. 23/bp $0. The phosphoramidite method hasn’t changed over the past three decades. Eurofins Genomics is an leading provider of DNA/RNA oligonucleotides, DNA sequencing services, Gene Synthesis products, Next Generation Sequencing services, genotyping services, and bioinformatics services for academic, agroscience,. 35/bp Request a quote Request a quote $0. Iron is an essential part of oxygen transport within the body and is present in hemoglobin, myoglobin, and the electron transport chain. SC1619 Cat No. 23/bp $0. Our customer service representatives are. SC1619 Cat No. In the solid-phase gene synthesis process, the molecules are separately synthesized and bound. In this study, our 125-member AAV2 variant library costs ~US$33,000 to build through a commercial de novo gene synthesis vendor (2. SC1575 GenPlusTM High-Throughput Gene Synthesis Cat No. Cholesterol synthesis and uptake pathways are regulated at the transcriptional level through classic end-product feedback inhibition of 3-hydroxy-3-methylglutaryl (HMG) CoA reductase, the rate. and even genes longer than 8 kb through GenBrick™ Gene Synthesis. The human genome also includes noncoding sequences (e. However, there are three main differences between DNA and RNA: RNA uses the sugar ribose instead of deoxyribose. SC1584 $0. SC1010 Rush Gene Synthesis Cat No. It utilizes iterative restriction enzyme digest and sticky-end alignment to build a target sequence from a reference sequence. Figure 11. a. mRNA was then synthesized. 23/bp $0. Figure 3. Utilize the best-in-class codon optimization algorithm to optimize your sequence Select upgraded gene synthesis, cloning, plasmid DNA preparation, and add-on services for your applications Receive estimated pricing and turnaround time Get an instant quote and place your order GenScript's Plasmid DNA Preparation Service is accommodating to both small research labs as well as large-scale manufacturing biotech and pharmaceutical companies, providing you with high quality plasmids with 100% full insert sequence accuracy. 1950 DNA polymerase isolated from E. 45/bp. GenSmart™ Codon Optimization. 45/bp. Starting at $0. , Piscataway, NJ 08854, US. GenScript Launches Enhanced GenBrick Gene Synthesis Service for Building Synthetic Genomes. * Sequence verification of full gene insert only applies when bundled with gene synthesis (an insert to be cloned into a plasmid). 35/bp Request a quote Request a quote $0. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; however, plasmids. There are several methods for preparing siRNA, such as chemical synthesis, in vitro transcription, siRNA expression vectors, and PCR expression cassettes. Nov. SC1584 $0. DNA is the information molecule. Transcription is the first step of gene expression. Unlike large cDNA libraries that may contain partial or mutated sequences, mini ORF libraries are delivered as fully sequence verified, ready-to-express clones. 35/bp Request a quote Request a quote $0. 45/bp.